|ชื่อเรื่อง||Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms|
|ผู้แต่ง||S.M.S.D. Ramanayake and K. Yakandawala|
|บรรณานุกรม||Ramanayake, S.M.S.D. and K. Yakandawala. 1997. Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Science. 129(2) : 213-223.|
Propagules of Dendrocalamus giganteus were produced by a process of in vitro axillary shoot proliferation followed by rooting. Axillary bud break and fhe presence of culture contaminants in single-node segments of secondary branches were strongly influenced by the seasonal rainfall pattern. Cultures of axillary shoots were initiated during peak bud-break periods in a semi-solid MS medium with 2 mg 1- I benzylaminopurine (BAP), 0.1 mg 1- I kinetin and I g 1- I Benlate (benomyl). Continuous shoot proliferation for a period of 1 year was achieved in a liquid MS medium with 6 mg 1- I BAP, 0.1 mg 1- I kinetin and 8% (vjv) coconut water. Shoot proliferation at a 1.8-fold increase in shoot number every 13 days took place after a lag phase of 65 days. Shoots (77.5%) rooted when transferred from the shoot proliferation medium containing 3 mg 1- I indole butyric acid in the last two passages, to a rooting medium with MS modified to half strength major salts, 3 mg 1- I IBA and 10 mg 1- I coumarin. After a period of in vitro hardening the propagules were transferred to soil.